Technical Data
P3113-90A
Paxillin (PXN)
Description:
Paxillin can be diffusely distributed in the cytoplasm, co-localized with actin at the cell periphery, or aggregated at focal adhesions. Paxillin has several motifs which allow its interaction with other proteins. These motifs include LD motifs, LIM domains, an SH3 domain binding site, and SH2 domain binding sites. Paxillin complexes with proteins of focal adhesions including vinculin, talin, with the kinases FAK, PYK2, Src, and Csk, with ARF GTPase activating proteins, and with the adaptor protein v-Crk.

Paxillin is phosphorylated at several residues, including tyrosine 31, tyrosine 118, tyrosine 181. The phosphorylation of paxillin appears to be important for several biological functions including S-phase transition and neurite extension.

Applications:
Western blot: 1:1000–1:10,000
ELISA
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation

Control Lysate: A431 Lysate: Prepared from a human epidermoid carcinoma cell line. Cell lysate is available to be used as a positive control in Western blotting. Cell lysate is provided at a concentration of 1ug/ml in SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b- mercaptoethanol, 0.003% bromphenol blue and 5% glycerol). 5–10ul/lane is sufficient for Western blot
TypeIsotypeCloneGrade
MabIgG12Q1106Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceChickenMouse
Concentration:
~1mg/ml
Immunogen:
Affinity purified phosphotyrosine-containing proteins from Rous sarcoma virustransformed chick embryo fibroblasts
Purity:
Purified IgG
Form
In 50% glycerol, 20mM sodium phosphate, pH 7.5, 150mM sodium chloride, 1.5mM sodium azide.
Specificity:
Reacts strongly with two bands on Western blots of total cell lysate from chick embryo fibroblasts or rat brain. The lower molecular band is believed to be either an isoform or a degradation product of the 68kD protein, since it is found also on SDS-PAGE of affinity purified paxillin. Recognizes human, canine, mouse, chick and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. J. Biol. Chem. (1989). 264:20163–20166. 2. J. Cell Biol. (1989). 108:2401–2408. 3. J. Cell Biol. (1990). 111:1059–1068. 4. J. Biol. Chem. (1995). 270:3278–3283.