Technical Data
Paxillin (PXN)
Paxillin is a multi-domain protein that localizes primarily in focal adhesion sites to the extracellular matrix (1). Paxillin is one of the key components of integrin signaling and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at tyrosine 118 (3,4). Phospho-Tyr118 may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the
SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).

Suitable for use in Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide (KLH coupled) corresponding to residues near the carboxy- terminal sequence of human paxillin
Protein A and peptide affinity chromatography
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol
Detects endogenous levels of paxillin from various cell lines. Does not crossreact with other related proteins
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Turner, C.E. (2000) J. Cell Sci. 113, 41394140. (2) Burridge, K. et al. (1992) J. Cell Biol. 119, 893903. (3) Bellis, S.L. et al. (1995) J. Biol. Chem. 270, 1743717441. (4) Bellis, S.L. et al. (1997) Biochem. J. 325, 375381. (5) Schaller, M.D. and Parsons, J.T. (1995) Mol. Cell. Biol. 15, 26352645.