Technical Data
P3113-91B
Paxillin, phosphorylated, Tyr118 (PXN)
Description:
Paxillin is a 68kD cytoskeletal adapter protein involved in organization and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., actin, vinculin, Crk). Tyrosine 118, a Crk binding site of paxillin, is phosphorylated during integrin-mediated cell adhesion.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
NMuMG cells transfected with EGFP-tagged paxillin

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
10 Blots-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human paxillin that contains tyrosine 118.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity:
Recognizes mouse (93%) Paxillin when phosphorylated at Tyr118. Species sequence homology: human (100%) and chicken (87%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Manabe, R., et al. (2002) GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration. J. Cell Sci. 115(Pt 7):1497-1510 (cites the use of this antibody). Nikolopoulos, S.N. and C.E. Turner (2002) Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization. J. Biol. Chem. 277(2):1568-1575 (cites the use of this antibody). van de Water, B., et al. (2001) Suppression of chemically induced apoptosis but not necrosis of renal proximal tubular epithelial (LLC-PK1) cells by focal adhesion kinase (FAK). Role of FAK in maintaining focal adhesion organization after acute renal cell injury. J. Biol. Chem. 276(39):36183-36193 (cites the use of this antibody). Weinberg, J.M., et al. (2001) Energetic determinants of tyrosine phosphorylation of focal adhesion proteins during hypoxia/reoxygenation of kidney proximal tubules. Am. J. Pathol. 158(6):2153-2164 (cites the use of this antibody). West, K.A., et al. (2001) The LD4 motif of paxillin regulates cell spreading and motility through an interaction with paxillin kinase linker (PKL). J. Cell Biol. 154(1):161-176 (cites the use of this antibody). Schaller, M.D. and E.M. Schaefer (2001) Multiple stimuli induce tyrosine phosphorylation of the Crk-binding sites of paxillin. Biochem. J. 360(Pt 1):57-66 (cites the use of this antibody). Nakamura, K., et al. (2000) Tyrosine phosphorylation of paxillin _ is involved in temporospatial regulation of paxillincontaining focal adhesion formation and F-actin organization in motile cells. J. Biol. Chem. 275:27155 27164 (cites the use of this antibody). Salgia, R., et al. (1995) CRKL links p210BCR/ABL with paxillin in chronic myelogenous leukemia cells. J. Biol. Chem. 270(49):29145-29150.