Technical Data
Paxillin, phosphorylated (Ser126) (Paxillin alpha, PXN, PXN Protein, FLJ16691, FLJ23042)
Paxillin is a 68kD cytoskeletal adapter protein involved in organization and function of focal adhesions, which are critical to cell adhesion and migration. These cellular events in turn play a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, serine kinases (e.g., FAK, Pyk2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., actin, vinculin, Crk). Serine 126 of paxillin is phosphorylated as a result of Raf stimulation, through the Raf --> MEK --> ERK pathway.

Positive Control: RAW 264.7 cells treated with LPS; 293 cells transfected with wild-type EGFP-tagged human paxillin.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic phosphopeptide derived from the region of human paxillin that contains serine 126.
Purified by immunoaffinity chromatography.
As reported
Recognizes human Paxillin when phosphorylated at Ser126. Species Crossreactivity: Mouse (100% homologous) paxillin. Chicken (100% homologous) has not been tested, but is expected to react.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Woodrow, M.A., et al. (2003) Ras-induced serine phosphorylation of the focal adhesion protein paxillin is mediated by the Raf-->MEK-->ERK pathway. Exp. Cell. Res. 287(2):325-338.
Schaller, M.D. (2001) Paxillin: a focal adhesion-associated adaptor protein. Oncogene 20(44):6459-6472. Review.