		Technical Data
P3113-91F	Paxillin, ID (PXN)

Description:
PXN is a cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. PXN contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the Center region of human PXN (KLH).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human PXN.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Doan,A.T.,Exp. Cell Res. 313(12), 2667-2679 (2007). 2.Birukova,A.A.,Am. J. Physiol. Lung Cell Mol. Physiol. 293(1), L199-L211 (2007). 3.Gehmlich,K.,J. Mol. Biol. 369(3), 665-682 (2007). 4.Rathore,V.B.,Biochem. J. 403(2), 275-281 (2007).