Technical Data
P3113-91K
Paxillin, phosphorylated (Ser126) (Paxillin alpha, PXN, PXN Protein, FLJ16691, FLJ23042)
Description:
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Pervandate treated and untreated Huvec cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human Paxillin at phosphorylated Ser126 (KLH).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS (no Ca2+, no Mg2+), pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide.
Specificity:
Recognizes human phospho-Paxillin (Ser126). Species crossreactivity: mouse. Sequence homology: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.