Technical Data
Paxillin, phosphorylated (Ser126) (Paxillin alpha, PXN, PXN Protein, FLJ16691, FLJ23042)
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Pervandate treated and untreated Huvec cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to human Paxillin at phosphorylated Ser126 (KLH).
Purified by affinity chromatography.
Supplied as a liquid in PBS (no Ca2+, no Mg2+), pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide.
Recognizes human phospho-Paxillin (Ser126). Species crossreactivity: mouse. Sequence homology: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.