		Technical Data
P3113-91S	Paxillin (PXN)

Description:
The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68kD focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	10K301	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Chicken	Mouse

Concentration:

As reported

Immunogen:

C-terminal part of recombinant chicken paxillin (aa305-559).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.

Specificity:

Recognizes chicken Paxillin. Species Crossreactivity: human, mouse, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.