Technical Data
P3113-91T
Paxillin, phosphorylated (Tyr118) (PXN)
Description:
Paxillin is a protein with four LIM domains, a proline-rich domain containing a consensus SH3-binding site, and three potential SH2-binding sites. It acts as an ERK-regulated scaffold that coordinates FAK and Rac activation in epithelial morphogenesis. Paxillin is a novel vinculin-binding protein present in focal adhesions and its isoforms in monocyte and cancer cells are known to have different binding properties to focal adhesion proteins. Paxillin binds to schwannomin and regulates its density-dependent localization and effect on cell morphology. Phosphorylation of Paxillin by JNK is known to regulate cell migration. The human Paxillin gene is localized in the chromosomal region 12q24.31.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.025-0.1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
20ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to human Paxillin protein containing a phosphorylated tyrosine residue at position 118.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human Paxillin (Y118) phosphorylated. Species Crossreactivity: chicken, chimpanzee, canine, mouse, rat, zebrafish. Species sequence homology: xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fernandez, C. et al. Nature. Genet. 31:354-362 (2002). 2. Huang, C. et al. Nature. 424:219-223 (2003). 3. Ishibe, S. et al. Molec. Cell. 16:257-267 (2004).