Technical Data
P3113-97
Pax2 (Paired Box Gene 2, Paired Box Homeotic Gene 2, Paired Box Protein 2, Paired Box Protein Pax-2, Paired Box Protein Pax2, Pax-2)
Description:
The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. The paired box containing genes were first identified in Drosophila and were subsequently described in a variety of species including mouse, human, chicken, Xenopus and Zebrafish. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6 is associated with Aniridia, and pax-2 which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms’ tumors. Persistent expression of Pax-2 in Wilms’ tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms’ tumor suppresser protein WT1 coincides with down-regulation of the pax-2 gene, and the WT1 protein can directly repress pax-2 transcription. Therefore, pax-2 is thought to be a target gene for WT1.

Applications:
Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 0.1-1ug/ml
Western Blot: 1ug/ml
Immunohistochemistry: 5ug/ml (whole mount staining)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRabbit
Concentration:
~0.25mg/ml
Immunogen:
A GST-Pax-2 fusion protein corresponding to aa188-385 from the C-terminal of mouse Pax2.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes mouse Pax2. Does not crossreact with other Pax family members. The major epitopes are located between aa204-373 of mouse Pax2. Two proteins are detected in mouse embryonic kidney lysates: a major band at 46kD (Pax2b) and a minor band at 48kD (Pax2a). Species Crossreactivity: ELISA: Immunogen; WB: Xenopus, zebrafish, embryonic mouse kidney, human Wilms’ tumors; IHC (frozen): rat, Xenopus, zebrafish, human Wilms’ tumor, mouse; IHC (paraffin): human Wilms’ tumor, mouse; IP: Embryonic mouse kidney.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dressler, G.R., et al., Proc. Natl. Acad. Sci. USA 89: 1179-1183 (1992). 2. Dressler, G.R., et al., Nature 362: 65-67 (1993). 3. Ryan, G., et al., Development 121: 867-875 (1995). 4. Gruss, P., et al., Cell 69: 719-722 (1992).