Technical Data
P3115-02A
PCNA (Proliferating Cell Nuclear Antigen)
Description:
Proliferating cell nuclear antigen (PCNA) is a 36kD nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfill several separate roles in the cell nucleus associated with changes in its antigenic structure.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (FFPE): 1:40 for biotin based detection. Pretreatment is not required. For optimal staining, incubate the primary antibody for 20-60 minutes at RT.
Optimal dilutions to be determined by the researcher.

Positive Comtrols:
Human colon or tonsil

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a6D645Affinity Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceRatMouse
Concentration:
Not Determined
Immunogen:
Recombinant rat PCNA (proliferating cell nuclear antigen).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.01M PBS, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes PCNA from all vertebrate species, insects and S. pombe. Intense nuclear staining of proliferating cells in normal and tumor tissues.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.