		Technical Data
P3115-08C	PCNA (Proliferating Cell Nuclear Antigen) (Biotin)

Description:
PCNA, originally known as Cyclin, is a 36kD nuclear protein associated with the cell cycle. It has recently been identified as the polymerase delta accessory protein. PCNA is essential for cellular DNA synthesis. It is also required for the in vitro replication of simian virus 40 (SV 40) DNA, where it acts to coordinate reading and lagging strand synthesis at the replication fork. This antibody is a useful tool for studying the proliferating cells in normal tissues, and possible aberrant expression in neoplasm. It is useful for staining the nuclei in proliferating calls during the G1, S, G2, and M stages. It has been suggested that high expression of PCNA correlates with organ metastasis and vascular invasion, and may be an effective prognostic factor (2).

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:5-1:20 (Alcohol or formalin-fixed, paraffin-embedded).
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice.

Recommended Positive Controls:
HeLa cells, A431 cells, WI-38 human fibroblasts, Xenopus cytosol and NIH 3T3 cells.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	3G236	Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.2mg/ml

Immunogen:

Recombinant PCNA polypetides.

Purity:

Purified

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide, 50% glycerol. Labeled with Biotin.

Specificity:

Recognizes human PCNA. Reacts with all vertebrate species, insects and S. pombe on Western Blots. Species Crossreactivity: mouse, rat, chicken, Xenopus.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Waseem, N.H. and Lane, D.P., J. Cell Sci. 96: 121 (1990). 2. Maeda, M.D., et al., Cancer 73: 528–533 (1994). 3. Cardoso, C., et al., Cell 74: 979–992 (1993). 4. Hall, P.A., et al., J. Pathol. 162: 285–294 (1990). 5. Robbins, B., et al., Arch. Pathol. Lab. Med. 111: 841 (1987). 6. Medrano, E.E., et al., Cancer Research 55: 4047–4052 (1995). 7. Pe’er, J., et al., Opthamology 101(1): 56–62 (1994). 8. Huang, G.Y., et al., J. Cell Biol. 143(6): 1725–1734 (1998). 9. Shpitz, B., et al., Human Pathology 29(5): 469–475 (1998). 10. Schneider, J., et al., Science 264:1467 (1994).