		Technical Data
P3115-11B	PCNA (Proliferating Cell Nuclear Antigen)

Description:
Proliferating cell nuclear antigen (PCNA) is a 36kD nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000. Detects a band at 30kD.
Immunohistochemistry (Paraffin Embedded): 10ug/ml. Heat-mediated citrate buffer antigen retrieval.
Optimal dilutions to be determined by the researcher

Postive Control:
DT40 B lymphocyte whole cell lysate (chicken)

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50% ) glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	3C169	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Rat	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Proliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein.

Purity:

Purified by Protein A chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. No preservatives added.

Specificity:

Recognizes rat PCNA p36 protein expressed at high levels in proliferating cells. Species crossreactivity: human, mouse, yeast (S. pombe and S. cerevisiae), chicken and Drosophila melanogaster.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Waseem NH and Lane DP (1990). J Cell Sci. 96 ( Pt 1), 121-129. 2.Hall PA, et al. (1990). J Pathol. 162(4), 285-294. 3.Landberg G, et al. (1991). 51(17), 4570-4574. 4.Woods AL, et al. (1991). Histopathology 19(1), 21-27, 29-33.