Technical Data
P3122-05
PDI (Protein Disulfide Isomerase)
Description:
The mammalian protein disulfide-isomerase (PDI) family encompasses several highly divergent proteins which are involved in the processing and maturation of secretory proteins in the ER by catalyzing the rearrangement of disulfide bonds (2,7). PDI, which is an abundant protein of the ER (> 400uM), has a carboxy-terminal retention signal sequence, KDEL, similar to that of BiP and Grp94 (1). The PDI proteins are characterized by the presence of one or more domains of ~95-110 amino acids related to the cytoplasmic protein thioredoxin (2). All but the PDI-D subfamily are composed entirely of repeats of such domains, with at least one domain containing and one domain lacking a redox-active-Cys-Xaa-Xaa-Cys-tetrapeptide (2). In addition to their roles as redox catalysts and isomerases, PDI proteins have other functions such as peptide binding, cell adhesion and perhaps chaperone activities (2). Platelet surface thiols and disulfides play an important role in platelet responses (3). Catalytically active PDI is found on platelet surfaces where it has been demonstrated to mediate platelet aggregation and secretion possible by reducing disulfide bonds thus leading to exposure of fibrinogen receptors in platelets (3).

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilutions:
Western Blot (Colorimetric): 1:1000
Optimal dilutions to be determined by researcher.

Positive Controls:
Purified Bovine PDI Protein
HeLa Cell Lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceBovineRabbit
Concentration:
~1mg/ml
Immunogen:
Purified bovine liver Protein Disulfide Isomerase (PDI).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Specificity:
Recognizes bovine PDI protein. Detects an ~58kD protein, corresponding to the apparent molecular mass of PDI on SDS-PAGE immunoblots. Species Crossreactivity: Human, mouse, rat, monkey, hamster, guinea pig, bovine, sheep, porcine, canine and Xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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