Technical Data
PDI (Protein Disulfide Isomerase, Cellular Thyroid Hormone Binding Protein, Disulphide Isomerase, DSI, ERBA2L, Glutathione Insulin Transhydrogenase, GIT, p55, PDIA1, PDIP, PDIR, PHDB, Procollagen-proline 2-oxoglutarate 4-dioxygenase beta, PO4DB, PO4HB, Pr
Protein disulfide isomerase (PDI) is an acidic 55kD thyroid hormone-binding protein that appears to be identical to the glial-p55 protein. It has been suggested to be involved in disulfide-bond formation and isomerization, as well as the reduction of disulfide bonds in proteins. It may also play a role in the thyroid hormone-dependent regulation of actin polymerization and the degradation of the membrane-associated protein type II iodothyronine 5'-deiodinase (5'D-II).

Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:5000-1:20,000
Immunohistochemistry (frozen): 1:100-1:1000
Immunohistochemistry (paraffin): 1:100-1:500
Western Blot: 1:10,000-1:20,000
Optimal dilutions to be determined by the researcher.

Cellular Localization: Endoplasmic reticulum

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceRatRabbit
Not determined
Purified full-length liver protein.
Supplied as a liquid (whole serum). No preservative added.
Detects rat PDI protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Safran, M. and Leonard, JL. Endocrinology. 129(4): 2011-2016 (1991)
2. Safran, M, et al. Endocrinology. 131(5): 2413-2418 (1992 [IHC, rat]