Technical Data
P3122-05A
PDI (Protein Disulfide Isomerase, Cellular Thyroid Hormone Binding Protein, Disulphide Isomerase, DSI, ERBA2L, Glutathione Insulin Transhydrogenase, GIT, p55, PDIA1, PDIP, PDIR, PHDB, Procollagen-proline 2-oxoglutarate 4-dioxygenase beta, PO4DB, PO4HB, Pr
Description:
Protein disulfide isomerase (PDI) is an acidic 55kD thyroid hormone-binding protein that appears to be identical to the glial-p55 protein. It has been suggested to be involved in disulfide-bond formation and isomerization, as well as the reduction of disulfide bonds in proteins. It may also play a role in the thyroid hormone-dependent regulation of actin polymerization and the degradation of the membrane-associated protein type II iodothyronine 5'-deiodinase (5'D-II).

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:5000-1:20,000
Immunohistochemistry (frozen): 1:100-1:1000
Immunohistochemistry (paraffin): 1:100-1:500
Western Blot: 1:10,000-1:20,000
Optimal dilutions to be determined by the researcher.

Cellular Localization: Endoplasmic reticulum

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
Not determined
Immunogen:
Purified full-length liver protein.
Purity:
Serum
Form
Supplied as a liquid (whole serum). No preservative added.
Specificity:
Detects rat PDI protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Safran, M. and Leonard, JL. Endocrinology. 129(4): 2011-2016 (1991)
2. Safran, M, et al. Endocrinology. 131(5): 2413-2418 (1992 [IHC, rat]