		Technical Data
P3122-11	PDI (Protein Disulfide Isomerase)

Description:
Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation on folding proteins (3).

Applications:
Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:100. Antigen retrieval in citrate buffer required.
Immunofluorescence (IF-IC): 1:50; Requires methanol permeabilization.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to residues surrounding Pro329 of human PDI.

Purity:

Purified by Protein A and peptide affinity chromatography.

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Specificity:

Recognizes endogenous levels of total human PDI protein at ~57kD. Species Crossreactivity: monkey, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Huppa, J.B. & Ploegh, H.L., Cell 92: 145-148 (1998). 2. Ellgaard, L. & Ruddock, L.W., EMBO Rep. 6: 28-32 (2005). 3. Tu, B.P. & Weissman, J.S., J. Cell Biol. 164: 341-346 (2004).