Technical Data
P3122-15
PDI (Protein Disulfide Isomerase)
Description:
Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulfide-bond formation and isomerization, as well as the reduction of disulfide bonds in proteins. PDI, which catalyses disulfide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulfide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulfide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94kD glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000; Detects a protein at 59kD representing PDI from rat liver extract or a slightly higher protein at 61kD representing PDI from human liver extract.
Immunoprecipitation: Shown to inhibit the activity of PDI in vitro and has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.
Immunohistochemistry (Frozen, FFPE): 1:100 Staining of rat intestine yields cytoplasmic staining.
Immunofluorescence: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a4i387Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Full length purified native rat PDI
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Specificity:
Recognizes rat PDI. Species Crossreactivity: human, mouse, porcine, hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.