		Technical Data
P3140-01	PEDF (Pigment Epithelial Derived Factor)

Description:
Pigment epithelium-derived factor (PEDF) was first identified as a 50kD secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells. More recently, PEDF was shown to have neurotrophic activity in the retina in vivo. PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells. The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. PEDF has been shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo.

Applications:
Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000 detects pigment epithelium on 10ug of human placenta lysate.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Positive Control:
Eye tissue

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	2Q1840	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant corresponding to aa196-347 of human PEDF fusion protein tagged with GST on the N-terminal was expressed in E. coli. Species Sequence Homology: rat.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.

Specificity:

Recognizes human PEDF at ~46kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Tombran-Tink, J., et al., 1991, Exp. Eye Res., 53: 411-414. 2. Cayouette, M., et al., 1999, Neurobiol. Dis., 6: 523-532. 3. Karakousis, P.C., et al., 2001, Mol Vis., 7: 154-163. 4. Dawson, D.W., et al., 1999, Science, 285: 245-248. 5. Stellmach, V., et al., 2001, Proc. Natl. Acad. Sci. USA, 98: 2593-2597.