Technical Data
P3340-50
Pericentrin 1 (PCNT1, PCN, PCNT, Pericentrin 1, Frount, Nucleoporin Nup75, Nucleoporin 85kD, NUP85)
Description:
Alternate Name: PCNT1, PCN, PCNT, pericentrin 1, frount, nucleoporin Nup75, nucleoporin 85kDa, NUP85

Domain Information: Chitinase

Gene Ontology Terms:

Application(s):
Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 75kD band observed in HeLa lysates (calculated MW of 75kD according to NP_079120). Recommended concentration: 1-3ug/ml.

1ug/ml staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See P3340-50-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
C-ARAIIREGSLEGS, C Terminus
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KD band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies). Species Crossreactivity: human, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Doxsey SJ, Stein P, Evans L, Calarco PD, Kirschner M. , Pericentrin, a highly conserved centrosome protein involved in microtubule organization., Cell. 1994 Feb 25;76(4):639-50.