Technical Data
P3340-50B
Pericentrin 1 (Pericentrin-1, PCNT1, FLJ12549, Frount, Frount Protein, Kendrin, KEN, Nucleoporin 85, Nucleoporin-85, Nucleoporin 85kDa, NUP85, Nucleoporin Nup75, Nup75, Pericentrin, PCN, PCNT)
Description:
NUP85 (Nucleoporin 85; also Nup75/nucleoporin 75 and FROUNT) is a 70-72kD member of the nucleoporin Nup85 family of proteins. It is one of nine members of the Nup107-160 subcomplex that performs at least two functions in the cell. First, it serves as an assembly point for nuclear pore formation, and may stabilize the bend that normally exists between the inner and outer nuclear membranes. Second, it appears to be quite stable, and during mitosis, promotes mitotic spindle assembly. Human NUP85 is 656aa in length. It is an a-helical protein with no obvious domain(s) or structural motif. Phosphorylation on Ser233, however, may regulate the overall interaction of the NUP107-160 subcomplex with the much larger nuclear pore. There one potential splice form that shows an alternative start site at Met47. Full-length NUP85 shares 92% aa identity with mouse NUP85.

Applications:
Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Immunocytochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanSheep
Concentration:
As reported
Immunogen:
E. coli-derived recombinant human Nucleoporin NUP85 (Accession # Q9BW27).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity:
Recognizes human NUP85. Species sequence homology: mouse (92%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.