Technical Data
P3340-53C
Pericentriolar Material 1 Protein, CT (PCM1, PCM-1, hPCM-1, PTC4)
Description:
PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.

Applications:
Suitable for use in ELISA, Immunohistochemistry, Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:50-1:100
Western Blot: 1:1000
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1816-1845 in the C-terminal region of human PCM1, UniProt Accession #Q15154.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human PCM1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Balczon R., Bao L.J. Cell Biol. 124:783-793(1994). 2. Corvi R., Berger N.Oncogene 19:4236-4242(2000). 3. Dammermann A., Merdes A.J. Cell Biol. 159:255-266(2002). 4. Reiter A., Walz C.Cancer Res. 65:2662-2667(2005). 5. Murati A., Gelsi-Boyer V.Leukemia 19:1692-1696(2005). 6. Hames R.S., Crookes R.E.Mol. Biol. Cell 16:1711-1724(2005).