Technical Data
P3340-99
Perilipin
Description:
Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, perilipin A and B arise by alternative RNA splicing from a single gene, generating polypepides of 57 and 46kD, respectively. The N-terminus, however, remains unchanged. The antiserum recognizes perilipins (A and B)
located at the surface of intracellular storage lipid droplets present, e.g. in the adrenal gland, adipocytes of white and brown adipose tissue, and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells.

Applications:
Suitable for use in Western Blot and Immunohistochemistry Other applications have not been tested.

Recommended Dilution:
Western Blot (ECL): 1:2000
Immunohistochemistry: 1:100-1:200 for 1 hour at RT on paraffin sections after microwave treatment or on frozen sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceGuinea pig
Concentration:
Not Determined
Immunogen:
Duplicated N-terminus of perilipin
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes perilipins (A and B) at ~57 and ~46kD, respectively. Does not cross-react with ADRP (adipophilin) or TIP47 proteins. Species Crossreactivity: human, rat and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Blanchette-Mackie EJ, et al. J Lipid Res 36(6), 1211-1226 (1995). 2. Souza SA, et al. JBC 273(38), 24665-24669 (1998). 3. Heid HW, et al. Cell Tissue Res 294, 309-321 (1998). 4. Martinez-Botas J, et al. Nat. Genet. 26, 474-479 (2000). 5. Wolins NE, et al. J. Biol. Chem. 276, 5101-5108 (2001). 6. Rydén M, et al. Biochem Biophys Res Com 318, 168-175 (2004). 7. Opretzka, ME: Topographie und Dynamik von Perilipin und Adipophilin in Lipidtropfen. PhD Thesis, University of Muenster/Germany (2005). 8. Forcheron F, et al. Arterioscler Thromb Vasc Biol 25, 1-7 (2005). 9. Yamaguchi T, et al. Mol Cell Biochem 284, 167-173 (2006). 10. Muthusamy K, et al. Immunohistochemical staining for adipophilin, perilipin and TIP47 in ocular sebaceous gland carcinoma. J Clin Pathol (2006).