Technical Data
P3342-01
Period Clock Protein 1 (dPER)
Description:
Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms. In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm. Human and mouse genes encoding a basic helix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported. The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291 amino acids respectively. Homologues of mPer1 designated Per 2 (1257aa) and Per3 (1113aa) have also been cloned. Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes. It has been suggested that mPer regulates neuronal activity in the SCN. Using genetic approach, a single mutation of A to T in the Clock gene affects circadian rhythmicity in mice. Clock has been mapped to chromosome 5. Mouse Clock encodes a transcription factor, a single polypeptide chain of 855aa (predicted calculated MW~97kD; pI 6.52; hClock, 846aa). Clock is abundantly expressed in brain (SCN, pyrifom cortex, hippocampus) as well as in other tissues (eye, total brain, tested, ovaries, liver, heart, lung, and kidney). Although Clock is constitutively expressed (not rhythmic) in the SCN, it may still be an important component of circadian machinery.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml. Using ECL
ELISA: 1:10,000-1:100,000. Using 50-100ng of P3342-01A control peptide/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceDrosophilaRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 21aa within the C-terminus of Drosophilla Period Clock Protein 1 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes Drosophilla Period Clock Protein 1. The peptide sequence has no sequence homology with mammalian PER or Clock proteins. The epitope is conserved in various alternatively spliced forms of dPer (dPerA, dPerB, etc.).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Grabek, K.R. and Chabot, C.C. (2012) Marine and Freshwater Behaviour and Physiology, DOI:10.1080/10236244.2012.730209 (WB). 1. Jackson, F.R., et al. Nature. 320: 185 (1986). 2. Reddy, P., et al. Cell. 46: 53 (1986). 3. Kilman, R.M., et al. Genetics. 133: 375 (1993). 4. Citri, Y, et al. Nature. 326: 42 (1987). Citations: Liu Y, 2004, Neuroscience, 130, 383-388 WB, IHC, 2. Zanello S B., 2000, J. Invest. Dermatol. 115: 757-760, IHC, 3. Uz T, 2003, NeuropsychoPharmacol. 28, 2117 2123, IHC, 4. Koyanagi S, 2005 Mol. Endocrinol., Nov 2005 WB 5. Gustincich S, 2004 PNAS. 101 , 5069-5074, IHC 6. Akhisaroglu M, 2004, Pharmacol. Biochem. Behavior 79, 37, WB 7. Weber F 2003, FEBS Lett., 555, 2, 341-345 WB 8. Yagita K 2001, Science, 292: 278 281, IF