Technical Data
P3342-01B
Period Clock Protein (dPER)
Description:
Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms. In Drosophila, the genes period (dPer) and timeless (tim), and Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm. Drosophila Period clock protein is involved in the generation of biological rhythms. It interacts with Timeless protein (Tim). dPER first accumulates in the perinuclear region about an hour before it is transported to the nucleus. It contains a remarkable run of alternating Gly-Thr residues.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000 using ECL
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
P3342-01A: Period Clock Protein 1 (dPER) Control Peptide

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20°CBlue IceDrosophilaRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to 21aa within the C-terminus of Drosophila Period Clock Protein 1, conjugated to KLH.
Purity:
Serum
Form
Supplied as a liquid with PBS, 0.05% sodium azide.
Specificity:
Recognizes Drosophilla Per. The epitope is conserved in various alternatively spliced forms of dPer (dPerA, dPerB, etc.). Species Crossreactivity: Drosophilla peptide sequence has no sequence homology with mammalian PER or Clock proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jackson, F.R., et al. (1986) Nature 320: 185. 2. Reddy, P., et al. (1986) Cell 46: 53. 3. Kilman, R.M., et al. (1993) Genetics 133: 375. 4. Citri, Y., et al. (1987) Nature 326: 42.