		Technical Data
P3342-02A	Period Clock Protein 1 (Period 1, Period-1, PER1, Period Circadian Protein Homolog 1, Period Drosophila Homolog of, Period Homolog 1, Circadian Clock Protein PERIOD1, Circadian Clock Protein PERIOD-1, Circadian Pacemaker Protein RIGUI, HGNC:8845, hPER, hP

Description:
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (IC): 1:10-1:2000
Western Blot: 1:200
Immunohistochemistry (Frozen, paraffin): 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to aa39-51, SLADDTDANSNG, from mouse Period Clock Protein 1. Cellular Localization: Cytoplasmic and nuclear

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide

Specificity:

Recognizes mouse Period Clock Protein 1, as well as recombinant mouse Period Clock Protein 1. Species Crossreactivity: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Darlington TK et al. Closing the circadian loop: CLOCK-induced transcription of its own inhibitors per tim. Science 280:1599-603 (1998). 2. Gekakis N et al. Role of the CLOCK protein in the mammalian circadian mechanism. Science 280:1564-9 (1998). 3. Reppert SM A clockwork explosion! Neuron 21:1-4 (1998).