Technical Data
P3342-11
Period Clock Protein 1 (PER1, RIGUI)
Description:
Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms. In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm. Recently human and mouse genes encoding a basic helix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported. The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291 amino acids respectively. Homologues of mPer1 designated Per 2 (1257aa) and Per3 (1113aa) have also been cloned. Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes. It has been suggested that mPer regulates neuronal activity in the SCN. Using genetic approach, a single mutation (A to T in the Clock gene affects circadian rhythmicity in mice.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Western Blot: 1-10ug/ml using ECL.
Immunohistochemistry: 2-20ug/ml
Immunofluorescence: 2-20ug/ml
Optimal dilutions to be determined by the researcher.

Control Peptide:
P3342-12A: Period Clock Protein 1, Mouse, Control Peptide

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to a 16aa sequence from mouse Period Clock Protein 1 conjugated to KLH. Epitope: ~C-terminal mapping downstream of the SG repeat of mouse Period Clock Protein 1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes mouse Period Clock Protein 1. No significant homology is seen with Per2, Per3, dPER or other known proteins. Species Sequence Homology: human and rat, 93%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Sun SZ et al (1997) Cell 90, 1003-1011. 2.Tei H et al (1997) Nature 389, 512-516.