Technical Data
P3342-20
Period Clock Protein 2 (PER2)
Description:
Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms. In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm. Recently human and mouse genes encoding a basic helix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported. The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291aa respectively. Homologues of mPer1 designated Per 2 (1257aa) and Per3 (1113aa) have also been cloned. Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes. It has been suggested that mPer regulates neuronal activity in the SCN. Using genetic approach, a single mutation (A to T in the Clock gene affects circadian rhythmicity in mice. Clock has been mapped to chromosome 5. Mouse Clock encodes a transcription factor, a single polypeptide chain of 855 aa (predicted calculated mol wt ~97kD; pI 6.52; hClock, 846aa). Clock is abundantly expressed in brain (SCN, pyrifom cortex, hippocampus) as well as in other tissues (eye, total brain, tested, ovaries, liver, heart, and kidney).

Applications:
Suitable for use in Western Blot, ELISA, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions:
Western Blot: 1-10ug/ml using ECL.
Immunohistochemistry: 2-20ug/ml.
Immunofluorescence: 2-20ug/ml
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide (Cat #P3342-20C)/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to 23aa near the C-terminal of human PER2, conjugated to KLH. Species Sequence Homology: rat, 77%
Purity:
Purified by immunoaffinity chromatography
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes mouse Per 2. No significant sequence homology with human Per2, Per1 or Per3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jilg A 2005 European J.Neurosc. 22, Issue 11: 2845-2854, Dec 2005 IHC. 2. Perrin JS, 2006, PNAS 103, 5591-5596. IHC. 3. Sugiyama T 2004 Biochem. Biphys. Res. Commun. 323, 479-483, IHC. 4. Field MD 2000 Neuron 25: 437 WB, IHC. 5. Nishii K 2006 Neuroscience Letters, 401, 44-48 WB. 6. Nuesslein-Hildesheim,Barbara 2000 Eur. J. Neurosci., 12, 2856-2864 WB, IHC. 7. Lamont EW T 2005 Neuroscience, 132, 245-248 IHC. 8. Yan L 2004 Eur. J. Neurosci. 19, 4, 1105-1109 IHC 9. Shigeyoshi Y 2002 Genes to Cells 7 2 163 IHC. 10. Bae Kiho 2000 Neuron 30, 525-536, IHC. 11. Castillo MR 2004 Am J Physiol Regulatory Integrative Comp Physiol, 287: 551 - 555. IHC. 12. Masubuchi S 2005 J. Neurosci., 25: 4719 - 4724. IHC. 13. Beaule C 2004 Neuroscience, 121, 2, 6 253-257, IHC. 14. Yagita K, 2001, Science, Apr 2001; 292: 278-281, IF. 15. Albretch U et al Cell 91:10551064 (1997); 16. Shearman LP et al Neuron 19:12611269 (1997); 17. Sakamoto K et al J Biol. Chem. 273:270392734 (1998) 18. Nagase T eat al (1997) DNA Res. 4:141150.