Suitable for use in ELISA, Western Blotting. Other applications not tested.
Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| As reported|
|A 23-aa peptide sequence within C-terminus of mouse Per2 (1) was synthesized coupled to KLH|
|Supplied as a liquid, neat serum, 40% glycerol.|
|The mouse peptide sequence is 77% conserved in rat Per2. No significant homology is seen with human Per2 or Per1 or Per3. Antibody crossreactivity with Per2 from other species is not known. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
(1). Albretch U et al (1997) Cell 91, 1055-1064; Shearman LP et al (1997) Neuron 19, 1261-1269; Sakamoto K et al (1998) J Biol. Chem. 273, 27039-27342. (2) Nagase T eat al (1997) DNA Res. 4, 141-150 (3) Field MD et al (2000) Neuron 25, 437; Bae K et al (2000) 30, 525-536; Nuesslein-Hildesheim B et al (2000) Eur. J. Neurol. 2856.