		Technical Data
P3345	Peripherin (PRPH, Neurofilament 4, NEF4)

Description:
Peripherin is a Class III intermediate filament subunit found in both the peripheral and central nervous systems, though it is concentrated, as its name suggests, in the neurons of peripherial ganglia and their processes.

Applications:
Suitable for use in Immunocytochemistry and Western Blot. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG	2Q135	Supernatant

Size		Storage	Shipping	Source	Host
300ul		4°C (-20°C Glycerol)	Blue Ice	Rat	Mouse

Concentration:

Not Determined

Immunogen:

Electrophoretically pure trp-E-peripherin fusion protein containing all but the 4 N-terminal amino acids of rat peripherin.

Purity:

Tissue culture supernatant.

Form

Supplied as a liquid.

Specificity:

Recognizes rat peripherin. Species Crossreactivity: mouse, human, porcine and bovine. Does not work on chicken or other more distantly related species, which may lack peripherin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Neuron (1995) 15:1245–1258. 2. J. Neurocytology (1996) 25:181–196. 3. J. Neuroscience Res. (1997) 47:300–310. 4. J. Cell Biology (1999) 144:435–446. 5. Garcia-Anoveros, J., et al., J. Neuroscience (2001) 21:2678–2686.