Technical Data
P3345-01A
Peripherin (PRPH, Neurofilament 4, NEF4)
Description:
Peripherin is a type III intermediate filament found initially in sensory neurons of the peripheral nervous system (PNS). It serves as a marker for autonomic and sensory neurons in the PNS and motor neurons in the CNS. Peripherin is a diagnostic marker in amyotrophic lateral sclerosis and certain neuronally-derived tumors. Brain injuries can also trigger expression of peripherin and the formation of peripherin accumulations in neurons that are normally silent for this gene. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Autoantibodies to peripherin are also frequently seen in the sera of patients with diabetes.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10,000
Immunocytochemistry: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant full length peripherin purified from E. coli
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity:
Recognizes Peripherin from human, rat, rabbit, cat, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wildner H, et al. dILA neurons in the dorsal spinal cord are the product of terminal and non-terminal asymmetric progenitor cell divisions, and require Mash1 for their development. Development. Jun 2006; 133(11): 2105-2113. 2. Chang L, et al. Assembling an intermediate filament network by dynamic cotranslation. J. Cell Biol. Feb 2006; 172(5): 747-758. 3. Anitha M, et al. GDNF rescues hyperglycemia-induced diabetic enteric neuropathy through activation of the PI3K/Akt pathway. J. Clin. Invest. Feb 2006; 116(2): 344-356.