Technical Data
P3345-01J
Peripherin (Neurofilament 4, PRPH, NEF4, PRPH1)
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Technical Data |
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P3345-01J |
Peripherin (Neurofilament 4, PRPH, NEF4, PRPH1) |
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Description: Peripherin is a member the intermediate filament protein family which includes desmin, peripherin and vimentin. It is found in neurons of the central nervous system and in sensory neurons of the peripheral nervous system and thus is a useful neuronal-specific marker. Peripherin mRNA can be alternatively spliced, producing 3 proteins having molecular weights of ~56kD (Per-56), ~58kD (Per-58), and ~61kD (Per-61). Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good marker for the ballooned axons seen in Lou Gehrig's disease and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:500-1:5000 Western Blot: 1:500-1:2500 Immunohistochemistry (frozen, formalin fixed paraffin embedded): 1:500-1:5000 Immunocytochemistry: 1:500-1:5000 Optimal dilutions to be determined by the researcher. Positive Control: Brain Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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