Technical Data
P3345-01Q
PRPH (Peripherin, Neurofilament 4, NEF4, PRPH1)
Description:
Peripherin is a 57kD Class III intermediate filament subunit found initially in sensory neurons of the peripheral nervous system, which gives the protein its name. Certain sensory neurons in the brain stem express Peripherin normally, as do a few cortical and hippocampal neurons. Interestingly, peripherin protein is upregulated in the lower motor neurons that degenerate in Amyotrophic Lateral Sclerosis (ALS), focusing attention on peripherin as a potential contributory factor to this serious human neurodegenerative disease. Peripherin is also expressed in certain neuroendocrine tumors and in the insulinproducing cells of the pancreas.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10,000
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Immunocytochemistry: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to human PRPH expressed in E. coli.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity:
Recognizes human PRPH. Species Crossreactivity: Rat, rabbit, feline, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.