Technical Data
P3345-03
Peripherin (PRPH, Neurofilament 4, NEF4)
Description:
Peripherin is a Class III intermediate filament subunit found in both the peripheral and central nervous systems, though it is concentrated, as its name suggests, in the neurons of peripheral ganglia and their processes. Antibodies to peripherin can be used in identifying, classifying, and studying neurons in the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrigs disease and some neuronally derived tumors. Peripherin is also a major component of the cytoskeleton of PC12 cells, a widely used model system for studies of neuronal differentiation. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin-RDS, a photoreceptor protein associated with retinal degeneration and blindness.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100
Immunohistochemistry: Frozen, unfixed sections
Immunohistochemistry: Formalin fixed paraffin embedded sections. Keep fixation time short, i.e., less than 10 minutes in formalin, followed by PBS wash.
Immunocytochemistry: 1:25-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k3C170Supernatant
SizeStorageShippingSourceHost
500ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant peripherin
Purity:
Tissue culture supernatant
Form
Supplied as a liquid containing sodium azide.
Specificity:
Recognizes human peripherin. Species Crossreactivity: Reacts with all mammalian species tested to date.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Errante, L., et al., J. Neurosci. Res. 37: 515-528 (1994).