Technical Data
P3345-04A
Peripherin (Neurofilament 4, PRPH, NEF4, PRPH1)
Description:
Peripherin is a member the intermediate filament protein family which includes desmin, peripherin and vimentin. It is found in neurons of the central nervous system and in sensory neurons of the peripheral nervous system and thus is a useful neuronal-specific marker. Peripherin mRNA can be alternatively spliced, producing 3 proteins having molecular weights of ~56kD (Per-56), ~58kD (Per-58), and ~61kD (Per-61). Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good marker for the ballooned axons seen in Lou Gehrig's disease and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:500-1:5000
Western Blot: 1:500-1:2500
Immunohistochemistry (frozen, formalin fixed paraffin embedded): 1:500-1:5000
Immunocytochemistry: 1:500-1:5000
Optimal dilutions to be determined by the researcher.

Positive Control:
Brain

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG111E316Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceRatMouse
Concentration:
As reported
Immunogen:
Recombinant corresponding to rat peripherin.
Purity:
Purified
Form
Supplied as a liquid, 0.01% thimerosal, 1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes rat Peripherin. Species Crossreactivity: feline, human, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Errante LD, Wiche G and Shaw G. Distribution of plectin, an intermediate filament-associated protein, in the adult rat central nervous system J. Neurosci. Res. 37:515-528 (1994). 2. Chien, C. L., Lee, T. H and Lu, K, S. Distribution of neuronal intermediate filament proteins in the developing mouse olfactory system. J. Neurosci. Res. 54:353-63 (1998). 3. Larivière, R. C., Nguyen, M. D., Ribeiro-da-Silva, A. and Julien, J.-P. Reduced number of unmyelinated sensory axons in peripherin null mice. J. Neurochem. 81:525-532 (2002). 4. He and Hays. Expression of peripherin in ubiquinated inclusions of amyotrophic lateral sclerosis. J. Neurol. Sci. 217:47-54 (2004). 5. Fasani F, Bocquet A, Robert P, Peterson A, Eyer J. 2004. The amount of neurofilaments aggregated in the cell body is controlled by their increased sensitivity to trypsin-like proteases. J Cell Sci. 2004 Feb 3;Pt. [Epub ahead of print] 6. Zhang X, Klueber KM, Guo Z, Lu C, Roisen FJ. 2004. Adult human olfactory neural progenitors cultured in defined medium. Exp Neurol. 186:112-123. 7. Lariviere RC, Julien JP. 2004.Functions of intermediate filaments in neuronal development and disease. J Neurobiol. 58:131-148.