Technical Data
P3352-21B
Peroxiredoxin 3 (Antioxidant Protein 1, PRDX3, Thioredoxin Dependent Peroxide Reductase)
Description:
Peroxiredoxin-3 is a ubiquitous, 22-28kD mitochondrial antioxidant enzyme that belongs to the 2-Cys class of the TSA/ahpC family of peroxiredoxins. Prx-3 is known to act as either a homodimer, or a decamer, and scavenge reactive oxygen species generated by oxidative stress. The mouse Prx-3 precursor molecule is 257aa in length. It contains a cleavable N-terminal 63aa mitochondrial targeting sequence, plus a 194aa mature enzyme that shows a thioredoxin domain between aa64-222. There are two catalytic cysteines, one at Cys109 and another at Cys230 of the precursor. Prx-3 undergoes a phosphorylation at Thr147 that reduces its activity. One potential splice form shows a deletion of Arg149, Lys150 and Arg185. Full-length mouse Prx-3 is 86-95aa identical to human and rat Prx-3, respectively.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseGoat
Concentration:
Not determined
Immunogen:
Recombinant corresponding to aa1-257 from mouse Peroxiredoxin3 expressed in E. coli (P20108).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity:
Recognizes mouse Peroxiredoxin 3. Species Crossreactivity: human. Species sequence homology: Recombinant mouse Peroxiredoxin 2 (6%). Recombinant human Peroxiredoxin 1, Peroxiredoxin 5, and Peroxiredoxin 6 (1%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.