Technical Data
PHD1 (PH1, prolyl hydroxylase domain-containing protein 1, HIFPH1, HIF-prolyl hydroxylase 1, EGLN2, EGL nine homolog 2, EIT6, estrogen-induced tag 6, DKFZp434E026)
Oxygen is absolutely critical for the survival of mammalian cells. Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits. However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e. by regulated stabilization of the alpha-subunits. Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564. This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome. Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation. The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1-3 or HPH1, HPH2, and HPH3). The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline.
PHD1 (human 407-aa, chromosome 19; also known as Egl nine homolog 2, EGLN2 (Hypoxia-inducible factor prolyl hydroxylase 1) (HIF-prolyl hydroxylase 1) (HIF-PH1) (HPH-3) (Prolyl hydroxylase domain-containing protein 1) (PHD1) (Estrogen-induced tag 6) is a hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. PHD1 is expressed abundantly in all tissues with highest expression in testis. It is expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000; Using 50-100ng of P3373-05A control peptide/well.
Western Blot: 1-5ug/ml using ECL technique.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
A synthetic 22aa peptide of the C-terminus human PHD1/EGLN2.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.2.
Recognizes human PHD1. Species Sequence Homology: mouse, rat - 95%. No significant sequence homology exist with other PHDs.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Taylor MS et al (2001) Gene 275, 125-132; Seth P et al (2002) Oncogen 21, 836-843; Strausberg RL et al (2002) PNAS 99, 16899-16903; Semenza GL et al (2001) Cell 107, 1-3; Epstein AC et al (2001) Cell 107, 43-54; Oehme F et al (2002) BBRC 296, 343-349