Technical Data
P3373-05
PHD1 (PH1, prolyl hydroxylase domain-containing protein 1, HIFPH1, HIF-prolyl hydroxylase 1, EGLN2, EGL nine homolog 2, EIT6, estrogen-induced tag 6, DKFZp434E026)
Description:
Oxygen is absolutely critical for the survival of mammalian cells. Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits. However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e. by regulated stabilization of the alpha-subunits. Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564. This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome. Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation. The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1-3 or HPH1, HPH2, and HPH3). The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline.
PHD1 (human 407-aa, chromosome 19; also known as Egl nine homolog 2, EGLN2 (Hypoxia-inducible factor prolyl hydroxylase 1) (HIF-prolyl hydroxylase 1) (HIF-PH1) (HPH-3) (Prolyl hydroxylase domain-containing protein 1) (PHD1) (Estrogen-induced tag 6) is a hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. PHD1 is expressed abundantly in all tissues with highest expression in testis. It is expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000; Using 50-100ng of P3373-05A control peptide/well.
Western Blot: 1-5ug/ml using ECL technique.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic 22aa peptide of the C-terminus human PHD1/EGLN2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes human PHD1. Species Sequence Homology: mouse, rat - 95%. No significant sequence homology exist with other PHDs.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Taylor MS et al (2001) Gene 275, 125-132; Seth P et al (2002) Oncogen 21, 836-843; Strausberg RL et al (2002) PNAS 99, 16899-16903; Semenza GL et al (2001) Cell 107, 1-3; Epstein AC et al (2001) Cell 107, 43-54; Oehme F et al (2002) BBRC 296, 343-349