Technical Data
P3373-05D
PHD1 (Prolyl Hydroxylase Domain-containing Protein 1, PHD-1, DKFZp434E026, EGL Nine (C.elegans) Homolog 2, Egl Nine Homolog 2, EGLN2, Estrogen-induced Tag 6, EIT6, EIT-6, Hypoxia-inducible Factor Prolyl Hydroxylase 1, HIF Prolyl Hydroxylase 1, HIF-PH1, HI
Description:
PHD1 is a 44kD enzyme expressed abundantly in all tissues with the highest expression in testis.

Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 1 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.

Prolyl hydroxylase 1 might also play a role in the regulation of cell growth.

Applications:
Suitable for use in Immunohistochemistry (paraffin) and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100-1:250
Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.

Positive Control: Human testis

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a9i116Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Full length human PHD1 recombinantly expressed.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, HRP stabiliser, 0.01% thiomersal. Labeled with horseradish peroxidase (HRP).
Specificity:
Recognizes human PHD1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.