Technical Data
P3374-01D
PHD2 (Prolyl Hydroxylase Domain Containing Protein 2, PHD-2, Chromosome 1 Open Reading Frame 12, C1orf12, DKFZp761F179, ECYT3, EGL-9 Homolog of C. elegans 1, EGL Nine (C. elegans) Homolog 1, Egl Nine Homolog 1, EGLN1, HIF Prolyl Hydroxylase 2, HIF-PH2, HI
Description:
HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.
HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

Applications:
Suitable for use in Western Blot, Flow Cytometry, Immunoprecipitation and Immunohistochemistry.. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:100
Western Blot: 1:500-1:2500. Detects a band at 46-50kD
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C Do Not FreezeBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
Specificity:
Recognizes human PHD2. Recognizes an epitope mapped to a region between residues 1 and 50 of human PHD2 (NP_071334.1) (GeneID 54583). Does not react with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bruick, RK, McKnight, SL. A Conserved Family of Prolyl-4-Hydroxylases That Modify HIF. Science. 294: 1337-1340 (2001) 2. Ivan, M, et al. Biochemical Purification and Pharmacological Inhibition of a Mammalian Prolyl Hydroxylase Acting on Hypoxia-Inducible Factor. PNAS. 99(21): 13459-13464 (2002) 3. Maxwell, PH, Ratcliffe, PJ. Oxygen Sensors and Angiogenesis. Cell & Dev. Bio. 13: 29-37 (2002)