Technical Data
PHD2 (Prolyl Hydroxylase Domain Containing Protein 2, PHD-2, Chromosome 1 Open Reading Frame 12, C1orf12, DKFZp761F179, ECYT3, EGL-9 Homolog of C. elegans 1, EGL Nine (C. elegans) Homolog 1, Egl Nine Homolog 1, EGLN1, HIF Prolyl Hydroxylase 2, HIF-PH2, HI
HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.
HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

Positive Control: Mouse kidney lysate

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul-20CBlue IceMouseRabbit
A synthetic peptide made to an internal portion of the mouse PHD2 protein sequence (between residues 300-400).
Purified by immunoaffinity chromatography.
Tris-citrate/phosphate [pH 7-8]
Specific for PHD2. Species Crossreactivity: Reacts with mouse PHD2 protein. The immunogen used for this antibody production has 100% homology with rat protein. There is no significant homology with the human protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.