Technical Data
PHD3 (Prolyl Hydroxylase Domain-containing 3, PH3, Egl Nine Homolog 3, EGL9 Homolog of C. elegans 3, EGLN3, Factor Responsive Smooth Muscle Protein, FLJ21620, HIF-Prolyl Hydroxylase 3, HIFP4H3, HIFPH3, P4H3, SM-20)
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1: 8000
Western Blot: 0.1-0.3ug/ml
Optimal dilutions to be determined by the researcher.

Positve Control:
Human muscle lysates; ~30kD

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
As reported
Synthetic peptide correspoding to sequence C-RLGKYYVKERSK, from the internal region of the protein sequence. (NP_071356.1)
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Recognizes human EGLN3. Species sequence homology: Epitope is 100% homologous to human, mouse, rat, canine, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.