Technical Data
PHD4 (PH4, PH-4, Prolyl Hydroxlase Domain-containing 4, Prolyl Hydroxylase 4, HIF Prolyl Hydroxylase 4, HIFPH4, Hypoxia-inducible Factor Prolyl 4-hydroxylase, HIF Prolyl 4-hydroxylase, FLJ20262, Hypoxia Inducible Factor Prolyl Hydroxylase, P4H with Transm
Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.
Prolyl hydroxylase 4, specifically, catalyzes the post-translational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, may targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

Positive Control: HeLa and human glioblastoma cell lysates

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
A synthetic peptide made to a portion of the C-terminus of the human prolyl hydroxylase domain-containing 4 encoded within exon 4. [LocusLink 54681].
Purified by peptide immunoaffinity chromatography.
Tris-citrate/phosphate [pH 7-8]
This antibody is specific for prolyl hydroxylase 4. Species Crossreactivity: Human. Does not appear to work in mouse. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bruick, RK and McKnight, SL. A Conserved Family of Prolyl-4-Hydroxylases That Modify HIF. Science. 294: 1337-1340 (2001)
2. Ivan, M, et al. Biochemical Purification and Pharmacological Inhibition of a Mammalian Prolyl Hydroxylase Acting on Hypoxia-Inducible Factor. PNAS. 99(21): 13459-13464 (2002)
3. Maxwell, PH and Ratcliffe, PJ. Oxygen Sensors and Angiogenesis. Cell & Dev. Bio. 13: 29-37 (2002)