Technical Data
Media
Peptone Water
P3505-50
Microbiological Grade
Storage 4C    Shipping RT
Formula (Grams per liter may be adjusted or formula supplemented to obtain desired performance) per Liter:
Peptone10.0 g
Sodium Chloride5.0g
Final pH: 7.2 0.2 at 25.0C
Peptone Water is a broth medium used for the detection of indole and carbohydrate fermentation patterns of non-fastidious organisms. Peptone Water is a minimal growth medium which is used for determining carbohydrate fermentation capabilities.

Recommended Dilutions:
Carbohydrate Fermentation Study: 1% final carbohydrate concentration with phenol red (0.018g/L) indicator and Durham tube. After incubation a yellow broth with or without gas bubbles indicates a positive fermentation reaction.
Indole Test: Place an indole test strip 10mm above a 24-48 hr. broth culture. Reincubate for 5-30 minutes. A violet red color on the test strip indicates a positive reaction.

Storage and Stability:
Store unopened medium at 2-30C. Store opened medium container in a low humidity environment at 2-30C. Store liquid medium at 4C. The powder medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.


Directions per Liter Dissolve 15.0 grams per liter of distilled/deionized water, heating with stirring until completely solubilized, adjusting pH as necessary. If required, add sodium bicarbonate with stirring when solution has cooled. Sterilize with 0.2 membrane filter. Aliquot into sterile containers.

Storage Store at 0-5C (store unopened media at 2-30C). Opened bottles should be capped tightly and kept in a dark, low humidity environment.

Appearance: Light beige, homogeneous, free flowing powder Solubility

Microbiological Analysis: Escherichia coli (ATCC 25922)


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.