Phenol Red Broth Base (Powder)
Storage RT/4°C Shipping RT
Components per Liter (g/Liter)
A basal medium to which carbohydrates are added to observe fermentation reactions.
Mode of Action:
Fermentation of the added reactant by the inoculated culture causes phenol red to change its color from red to yellow. When testing anaerobes, addition of a small quantity of agar stabilizes the anaerobiosis.
Typical Quality Control:
Staphylococcus aureus ATCC 25923
Enterococcus faecalis ATCC 11700
Klebsiella pneumoniae ATCC 13883
Proteus vulgaris ATCC 13315
Shigella flexneri ATCC 12022
Salmonella typhimurium ATCC 14028
Directions per Liter: Dissolve 16 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. and boil for one minute to completely dissolve the medium. If required, add carbohydrates (5-10 grams). Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Appearance: Light to medium beige, homogeneous, free flowing powder
Solubility: Red, clear, complete
pH: As Reported
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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