Technical Data
P4070-87
PHLPP (PH Domain and Leucine-rich Repeat Protein Phosphatase, PH Domain Leucine-rich Repeat Protein Phosphatase, PH Domain Leucine-rich Repeat-containing Protein Phosphatase, PHLPP1, KIAA0606 Protein, Pleckstrin Homology Domain-containing Family E Protein
Description:
Located on chromosome 18 and called PH domain Leucine-rich repeat Protein Phosphatase (PHLPP), the tumor suppressor is described in the April 1, 2005 issue of the journal Molecular Cell. The scientists demonstrated that PHLPP deletes a phosphate molecule, causing termination of cell-growth signaling by a protein called Akt that controls the balance between cell growth leading to cancer and cell death that prevents tumor formation. PHLPP levels are markedly reduced in several colon cancer and gliobastoma human cell lines that had elevated Akt phosphorylation.

Applications:
Suitable for use in Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
The epitope recognized maps to a region between residues 1050 and 1100 of human PH Domain and Leucine Rich Repeat Protein Phosphatase using the numbering given in entry NP_919431.1 (GeneID 23239).
Purity:
Purified by immunoaffinity chromatography.
Form
Tris-citrate/phosphate buffer with 0.09% Sodium Azide
Specificity:
Species Crossreactivity: This antibody crossreacts with human. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.