		Technical Data
P4070-87C	PHLPP (PH Domain and Leucine-rich Repeat Protein Phosphatase, PH Domain Leucine-rich Repeat Protein Phosphatase, PH Domain Leucine-rich Repeat-containing Protein Phosphatase, PHLPP1, KIAA0606 Protein, Pleckstrin Homology Domain-containing Family E Protein

Description:
PHLPP1 is the protein phosphatase responsible for dephosphorylation of the Akt (Ser473) site. Akt (Ser473) is the TORC2 (mTOR) phosphorylation activation site.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1,000
Optimal dilutions to be determined by the researcher.

Positive Control:
Human brain tissue lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Linear peptide corresponding to the C-terminus of PHLPP1 (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes human PHLPP1. Species Crossreactivity: mouse, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.