Technical Data
P4070-87H
PHLPP, NT (PH Domain and Leucine Rich Repeat Protein Phosphatase 1, PH Domain Leucine-rich Repeat-containing Protein Phosphatase 1, PHLPP1, KIAA0606, MGC161555, Pleckstrin Homology Domain-containing Family E Member 1, PH Domain-containing Family E Member
Description:
PHLPP1 is a protein phosphatase that specifically mediates dephosphorylation of 'Ser-473' of AKT1, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide selected from the N-terminal region of human PHLPP1 (KLH).
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human PHLPP1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Gao,T., J. Biol. Chem. 283(10), 6300-6311 (2008). 2.Brognard,J., Mol. Cell 25(6), 917-931 (2007). 3.Gao,T., Mol. Cell 18(1), 13-24 (2005).