Technical Data
P4071-14G2
PLAP (Phospholipase A-2-activating Protein, PLA2P, PLAA)
Description:
Anti-PLAP is a 70kD antibody, that reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placents during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:40 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissues sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.

Positive Control:
Placenta

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Recombinant corresponding to human PLAP.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Specificity:
Recognizes human PLAP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.