		Technical Data
P4071-16A	Phosphatase, Alkaline, Placental (Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, ALP, FLJ61142, PALP, Placental alkaline phosphatase 1, PLAP, PLAP-1)

Description:
Applications:
Suitable for use in Immunohistochemistry, ELISA
and Flow Cytometry. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 10ug/ml. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen sections; Not recommended for use on paraffin sections.
Optimal dilutions to be determined by the researcher.

Recommended Immunohistology Positive Control: Placenta, seminoma

Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control

Recommended Secondary Antibodies:
I1904-65V IgG, F(ab’)2 (FITC) Pab RbxMo
I1904-66A IgG, F(ab’)2, X-Adsorbed (FITC) Pab RbxMo
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	3H2834	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Placental membrane.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human placental alkaline phosphate, a 67kD isoform expressed in trophoblast cells of normal human placenta, in seminomas and in ovarian carcinoma. P4071-16A has been widely used in immunolocalization studies of germ cell tumors and may also be used in ELISA based assays for the measurement of soluble PLAP.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Fisken, J et al. (1989) J. Clin. Pathol. 42: 40-452. 2. Tucker, D. F. et al. (1985) Br. J. Cancer. 51: 631-639. 3. Epentos, A. A. et al. (1984) Br. J. Cancer. 49: 11-15. 4. Kalofonos, H. P. et al. (1990) J. Nucl. Med,. Allied. Sci. 34: 294 298. 5. Long, M.A. & Rossi, F.M. (2009) PLoS One. 4:e5435.