Technical Data
P4071-39
Alkaline Phosphatase
Description:
Alkaline phosphatase (AP) is a ubiquitously expressed enzyme which removes phosphate groups from target molecules, including DNA, RNA and alkaloids, under alkaline conditions, and is present at higher concentrations in the placenta (placental AP), intestines (intestinal AP) and liver/bone/kidney (tissue non-specific AP). In vivo studies have identified CIAP as a potential therapeutic agent for the treatment of lipopolysaccharide (LPS)-mediated inflammation and sepsis, due to the ability of CIAP to neutralise and thereby detoxify (LPS).

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:1000 -1:10000
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies
I1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceBovineRabbit
Concentration:
~5mg/ml
Immunogen:
Highly pure calf intestinal alkaline phosphatase.
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes calf intestinal alkaline phosphatase- (CIAP), a membrane-bound hydrolase enzyme highly expressed by intestinal mucosa. Species crossreactivity: porcine. Detects a band of ~67kD in porcine microvillar membranes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Braccia, A. et al. (2003) Microvillar membrane microdomains exist at physiological temperature. Role of galectin-4 as lipid raft stabilizer revealed by "superrafts". J. Biol. Chem. 278: 15679-15684.
2. Beumer, C. et al. (2003) Calf intestinal alkaline phosphatase, a novel therapeutic drug for lipopolysaccharide (LPS)-mediated diseases, attenuates LPS toxicity in mice and piglets. J. Pharmacol. Exp. Ther. 307: 737-744.