Technical Data
P4071-99D
Phosphodiesterase 1C, CT (PDE1C, Calcium/Calmodulin-Dependent 3',5'-Cyclic Nucleotide Phosphodiesterase 1C, Cam-PDE 1C, hCam-3)
Description:
Phosphodiesterase 1C (PDE1C) is a subtype of the PDE1 enzyme. PDE1C is activated by calmodulin binding in the presence of calcium, and degrades the cyclic nucleotides cAMP and cGMP. A number of PDE1C isoforms exist, each with different affinities for cAMP and cGMP. They have a range of molecular weights from 65-70kD. PDE1C is a major regulator of smooth muscle cell (SMC) proliferation in humans, and plays an important role in the development of organs containing smooth muscle. PDE1C may also have a role in olfaction, regulation of sperm function and neuronal signaling.

Inhibition of PDE1C may prove beneficial in the treatment of diseases such as atherosclerosis and pulmonary hypertension where excessive SMC proliferation occurs.

Applications:
Suitable for use in Immunohistochemistry (paraffin). Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 2-10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control Tissue:
Thyroid

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminal domain of human phosphodiesterase 1C (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH7.7, 1% BSA, 0.01% sodium azide.
Specificity:
Recognizes an epitope within the C-terminal region of human PDE1C. Species sequence homology: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rybalkin, S.D. et al. (2003) Cyclic GMP phosphodiesterases and regulation of smooth muscle function. Circ. Res. 93: 280-291. 2. Dolci S. et al. (2006) Subcellular localization and regulation of type-1C and type-5 phosphodiesterases. Biochem. Biophys. Res. Commun. 341: 837-846. 3. Yan, C. et al. (1996) The Calmodulin-dependent Phosphodiesterase Gene PDE1C Encodes Several Functionally Different Splice Variants in a Tissue-specific Manner. J. Biol. Chem. 271: 25699-25706.