Technical Data
P4072-01D
Phosphodiesterase 2A, NT (PDE2A, cGMP-Dependent 3',5'-Cyclic Phosphodiesterase, Cyclic GMP-Stimulated Phosphodiesterase, CGS-PDE, cGSPDEm)
Description:
PDE2A is a 100kD cyclic nucleotide phosphodiesterase (PDE). Three isoforms of PDE2A have been reported (PDE2A1, PDE2A2, and PDE2A3) which arise from a single gene by alternative splicing events. PDE2A can degrade both cAMP and cGMP, but degrades cGMP with greater efficacy.

PDE2A is expressed in a variety of tissue types, with greatest expression in the brain, where it is localised in the cerebellum and neocortex. PDE2A has been implicated in penile erectile dysfunction and in the regulation of fluid and inflammatory cell transport across the endothelial cell barrier.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 15-25ug/ml
Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher.

Positive Control Tissue:
Kidney

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to the N-terminal region of human phosphodiesterase 2A (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH7.7, 0.09% sodium azide.
Specificity:
Recognizes an epitope within the N-terminal (NT) region of human phosphodiesterase 2A. Species Crossreactivity: rat. Does not cross react with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sadhu, K. et al. (1999) Differential Expression of the Cyclic GMP-stimulated Phosphodiesterase PDE2A in Human Venous and Capillary Endothelial Cells. J. Histochem. Cytochem. 47: 895-905.